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The Phaseolus coccineus EST Project

Beans! We are using the giant embryos of Phaseolus coccineus (Scarlet Runner Bean) to identify genes active in different regions of post-fertilization plant embryos in order to understand the mechanisms responsible for cell differentiation events during early embryogenesis. Globular-stage embryos were dissected from developing seeds and embryo proper (ep) and suspensor (s) regions were collected. cDNA libraries were generated from the suspensor and embryo-proper total RNAs. The 5' ends of individual cDNA clones from each library were subjected to "single-pass" high-throughput sequencing. To date, we have sequenced a total of 16,810 suspensor and 3,311 embryo-proper cDNAs. These ESTs have been categorized into different functional groups based on the results of BLAST searches and organized into an EST relational database. We identified ~250 ESTs representing different transcription factor classes (e.g., AP2/EREBP, B3 domain, CCAAT-box binding protein, homeodomain, polycomb complex, etc.) and ~250 signaling transduction proteins.

Our Scarlet Runner Bean EST database is open to the scientific community in order to examine mRNAs found in the suspensor and embryo proper of the globular-stage embryos. In addition, you can identify orthologs to your DNA sequence(s) by performing BLASTN and/or TBLASTX searches against our EST database.

Click here to browse or BLAST your sequences against the Phaseolus coccineus EST database.

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2003-07-11

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